Expression of Candida albicans SAP1 and SAP2 in experimental vaginitis
نویسندگان
چکیده
منابع مشابه
Analysis of secreted aspartic proteinases from Candida albicans: purification and characterization of individual Sap1, Sap2 and Sap3 isoenzymes.
The recently discovered secreted aspartic proteinase multi-gene (SAP) family in Candida albicans has complicated assessment of proteolytic activity as a factor in the onset and development of Candida infections. Differential expression of the SAP genes under various conditions, as well as possible variation in the properties of the individual isoenzymes, have consequences for immunological dete...
متن کاملDisruption of each of the secreted aspartyl proteinase genes SAP1, SAP2, and SAP3 of Candida albicans attenuates virulence.
Secreted aspartyl proteinases (Saps), encoded by a gene family with at least nine members (SAP1 to SAP9), are one of the most discussed virulence factors produced by the human pathogen Candida albicans. In order to study the role of each Sap isoenzyme in pathogenicity, we have constructed strains which harbor mutations at selected SAP genes. SAP1, SAP2, and SAP3, which are regulated differentia...
متن کاملEvaluation of Candida Vaginitis Epidemic Caused by Candida Albicans in Women of Kazerun City
Introduction: Candida albicans is one of the most common causes of vaginitis and one of the most important reasons for women to visit health centers. The aim of this study was to determine Candida albicans infection and its related factors in women in Kazerun. Methods: This cross-sectional study was performed in 2017 on 150 samples extracted from the laboratory. Swab specimens were prepared fr...
متن کاملExposure of Candida albicans to antifungal agents affects expression of SAP2 and SAP9 secreted proteinase genes.
OBJECTIVES To ascertain the effects of subinhibitory concentrations of several antifungal agents on a virulence factor: secreted proteinase (Sap) activity and expression of SAP genes in Candida albicans. METHODS Enzyme assays and growth measurements, GFP-SAP2 promoter constructs and fluorescence measurement, transcript profiling and RT-PCR. RESULTS For seven of eight C. albicans isolates te...
متن کاملSecretory Aspartyl Proteinases Cause Vaginitis and Can Mediate Vaginitis Caused by Candida albicans in Mice
UNLABELLED Vaginal inflammation (vaginitis) is the most common disease caused by the human-pathogenic fungus Candida albicans. Secretory aspartyl proteinases (Sap) are major virulence traits of C. albicans that have been suggested to play a role in vaginitis. To dissect the mechanisms by which Sap play this role, Sap2, a dominantly expressed member of the Sap family and a putative constituent o...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1995
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.63.5.1887-1892.1995